Fig. 7. H2N2 cross-reactive T cells are present in human blood donors that were born after H2N2 stopped circulating.

Frozen PBMCs from healthy human donors (n = 18) were thawed and stimulated with peptide pools coding for the internal proteins of H2N2 or HIV gag in an ELISpot assay. Responses are corrected for background signal (minus medium stimulation). Boxplots depict the 25, 50, and 75% quantile where the upper and lower whiskers extend to the smallest and largest value respectively within 1.5* the inter quartile ranges.