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. 2020 Oct 9;10:16944. doi: 10.1038/s41598-020-74101-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of furin cleavage efficiency of different SARS-CoV2 and MERS-CoV S1/S2 cleavage site mutants. (a) Alignment of SARS-CoV2 and MERS-CoV S1/S2 regions with the mutants used in substrate constructs for analysis of furin cleavage specificity in panel ’b’. (b) The furin cleavage efficiency of different mutants was analyzed in an in vitro time-course experiment. The proteolytic cleavage of S1/S2-GB1 reporter constructs by furin is visualized by Coomassie staining of SDS-PAGE gels. Uncropped images are shown in Supplementary Fig. S1. (c) The plot shows the accumulation of cleaved S1/S2-GB1 reporter protein in an in vitro furin activity assay. The error bars show standard deviation.