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. 2020 Oct 9;11:5099. doi: 10.1038/s41467-020-18865-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — a, b Representative immunoblots (a) and relative protein levels (b) for mitochondrial uncoupling protein UCP1 in differentiation assay of inguinal white adipose tissue (iWAT) SVF cells treated with vehicle (n = 21), lactate (n = 21), MCT1/2 inhibitor (n = 6) or both (n = 6). c, d Representative immunoblots (c) and relative protein levels (d) for UCP1 in differentiation assay of 3T3-L1 preadipocytes treated with vehicle (n = 8), lactate (n = 8), MCT1/2 inhibitor (n = 4) or both (n = 6). e, f Representative immunoblots (e) and relative protein levels (f) for UCP1 in differentiation assay of 3T3-L1 preadipocytes treated with unfiltered serum (10%) from WT (n = 11) or YS (n = 11) mice. g, h Representative immunoblots (g) and relative protein levels (h) for UCP1 in differentiation assay 3T3-L1 preadipocytes treated with 10 kb-filtered serum (20%) from WT and YS mice, with (WT, n = 12, YS, n = 11) or without (WT, n = 6, YS, n = 6) the MCT1/2 inhibitor. P values are indicated as analyzed by ordinary one-way analysis of variance (ANOVA) with Dunnett’s multiple comparisons test (b, d, h), Welch’s t test (f). All statistical tests are two-sided. Data are represented as mean ± standard deviation. Relative protein levels are determined as protein levels normalized to vehicle (a, b) or wild-type mouse serum (f, h) treated controls. Source data are provided as a source data file.