Fig. 2.

PHF8 regulates the expression of genes involved in cell migration and cell motility. (A) DAVID functional annotation analysis of the genes with twofold or less alterations in PHF8-knockdown MKN28 in a microarray analysis. (B) qRT-PCR analysis of down-regulated genes in the cell migration/cell motility/cell motion category. The data were normalized by GAPDH mRNA levels. (C) ChIP analysis of PHF8 occupancy on the promoter region of genes involved in cell migration category in MKN28 using IgG or anti-PHF8 antibodies. (B and C) Data are presented as the average of three replicates ± SD *P < 0.05, **P < 0.01 (two-tailed Student's t test).