Fig. 4.

IAV infection promotes innate inflammation via the infiltration of monocytes and neutrophils in the aorta of pregnant mice. Single-cell suspensions were prepared from whole thoracic aorta digests from pregnant mice inoculated with either PBS or Hk-x31 virus (X-31; 10⁴ PFU) at 3 dpi (D3) and 6 dpi (D6) and quantified for the following cell subsets via flow cytometry. Representative dot plots and quantification are shown. (A) Patrolling monocytes (CD11b⁺Ly6C^low) and proinflammatory monocytes (CD11b⁺Ly6C^high). (B) Ly6G⁺ neutrophils and CD69⁺ activated neutrophils. All cell populations are measured as absolute number of CD45⁺ population per 25,000 counting beads. Data are represented as mean ± SEM (pregnant PBS, n = 5 or 6; pregnant X-31, n = 5 or 6; of at least two independent experiments). Statistical analysis was performed by using unpaired t test against their respective PBS control. *P < 0.05; **P < 0.01; ***P < 0.001.