Fig. 3.

Cys derivations and codon usage in δ-HXTXs. (A) Alignment of δ-HXTXs representing major changes in the Cys framework. (B) Expected and experimentally determined molecular masses for the seven Cys residue derivations of δ-HXTX homologs from H. infensa. Several masses closely matching either monomers or homo/heterodimers were observed using MALDI or Orbitrap mass spectrometry. (C) Codon usage for Cys residues in δ-HXTXs from different funnel-web genera. Lines at the top represent the four disulfide bonds in the prototypical Ar1a toxin.