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. 2020 Sep 23;117(40):25150–25158. doi: 10.1073/pnas.2011152117

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Copyright © 2020 the Author(s). Published by PNAS.

This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND).

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Fig. 2. — Subcellular localization of PICALM1. (A) Subcellular localization of GFP-VAMP721 and PICALM1a-TagRFP in root epidermal cells observed by confocal microscopy. (B) Subcellular localization of PICALM1a-GFP and PICALM1b-TagRFP in root epidermal cells observed by confocal microscopy. (C) Localization of PICALM1a-GFP and CLATHRIN LIGHT CHAIN 2 tagged with mKO (CLC-mKO) near the PM observed by VIAFM. (D) Localization of GFP-VAMP721 and PICALM1b-TagRFP near the PM observed by VIAFM. The arrowheads indicate a dot bearing both GFP and TagRFP signals. (E) Quantification of colocalization between PICALM1a-GFP and CLC-mKO (Left) and GFP-VAMP721 and PICALM1b-TagRFP (Right) observed by VIAFM. The 90°-rotated CLC-mKO or PICALM1b-TagRFP images were used to test whether the detected colocalization was random or not. Wilcoxon signed rank test was used for statistical analyses. ***P < 0.001. n = 38 images for PICALM1a-GFP and CLC-mKO and 40 images for GFP-VAMP721 and PICALM1b-TagRFP. (Scale bars: 10 μm in A and B; 5 μm in C and D.)