Table 2.

Cycle threshold (Ct) values and calculations for relative fold change in the expression of the actin-1 gene.

Samples	Ct test	Mean Ct (test)	Ct ref (GAPDH)	Mean Ct (ref)	∆Ct test (mean Ct of test‐ mean Ct ref)	∆∆Ct (∆Ct test‐∆Ct control)	2−∆∆Ct	SD	SEM
Infected A	15.87		18.93
	15.80	15.81	18.86	18.87	−3.06
	15.77		18.82
						−2.28	4.85	0.0573	0.033
Control A	16.33		17.12
	16.28	16.25	17.08	17.04	−0.79
	16.13		16.91
Infected B	15.6		18.47
	15.68	15.67	18.55	18.60	−2.87
	15.74		18.6
						−2.03	4.09	0.0132	0.008
Control B	15.78		16.62
	15.85	15.82	16.68	16.65	−0.84
	15.82		16.66

A: Anopheles gambiae and B: Culex quinquefasciatus.