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. 2020 Oct 9;20:464. doi: 10.1186/s12870-020-02648-1

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Tissue specific expression profiles of BnNRT2 family genes as determined in RNA-seq and qRT-PCR assays. a Heat maps of relative expression from RNA-seq analysis of BnNRT2 expression in eight different tissues. PS: pistil, SM: stamen, OV: ovule, S: stem, L: leaf, R: root, SQ: silique, P: pericarp. b-d Summary of tissue specific expression of BnNRT2 genes determined in qRT-PCR assays. b Expression of BnNRT2s of different tissues in adult plants at different growth stages. Each tissue of adult plants were collected from field plots supplemented with N supplied as 180 kg/hm² urea, in detail, junior leaf (JL), senior leaf (SL) and root (R) were collected at vegetative growth stage (namely over-wintering stage), flower (F) and stem (S) were collected at flowering stage, then seed (SD) and pericarp (P) were collected at reproductive growth stage. c The expression of BnNRT2.5 s in seedling tissues (21-days-old rapeseed seedlings). Rapeseed seedlings were reared in hydroponics cultures supplied with normal N treatment (9.5 mM N), L: leaf, R: root. d Expressions of BnNRT2.5 s and BnNRT2.7 s in the flower (F), stem (S), seed (SD) and pericarp (P) though qRT-PCR assays were further delineated in heat maps