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. 2020 Oct 9;20:464. doi: 10.1186/s12870-020-02648-1

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Responses of BnNRT2 expression profiles to alterations in N supply as determined in qRT-PCR assays. a Responses of root specific BnNRT2 genes (BnNRT2.1 s, BnNRT2.2a and BnNRT2.4a). b Responses of shoot specific BnNRT2 genes (BnNRT2.5 s and BnNRT2.7 s). c Responses of BnNRT2.5 s and BnNRT2.7 s in flower. d Responses of BnNRT2.5 s and BnNRT2.7 s in stem. e Responses of BnNRT2.7 s in seed. For these assays, rapeseed was cultivated in the field plots supplied with 180, 45 or 0 kg/hm² N. The rapeseed plants grown in 180 kg/hm² N-supplied plots were regarded as the control plots at each sampling time. Asterisks mark significant differences in expression between control plots and plots supplied 45 or 0 kg/hm² of urea (P < 0.05)