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. 2020 Oct 9;17:296. doi: 10.1186/s12974-020-01972-5

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Chronic alcohol induces inflammatory gene expression changes in multiple brain regions. a Proinflammatory cytokine proteins TNFα, IL-1β, and IL-6 were measured from the hippocampus of pair- (PF) and alcohol-fed (EtOH) as well as alcohol-fed mice treated with CVC for 3 weeks (EtOH +3wk CVC) by ELISA. b Inflammatory gene expression of pair- and alcohol-fed mice was analyzed using the Nanostring nCounter Immunology Panel and revealed 17 genes significantly altered in the cerebellum (green, expression increased; red, expression decreased). c Gene expression changes of genes found by Nanostring to be altered as well as some common inflammatory markers, and CCR2/5 axis genes were measured in the hippocampus, cortex, and cerebellum by qPCR in a larger cohort of pair- and alcohol-fed mice as well as mice treated with 3 or 6 weeks CVC (purple, > 3-fold increase in expression; cyan > 3-fold decrease in the expression compared to PF; complete gene expression profile data found in Tables 3, 4, and 5). Data are mean ± SEM, n = 4–6 mice/group. *p < 0.05 by one-way ANOVA