Fig. 6.

Alcohol downregulates the microglial expression of CD68. a Representative images of the hippocampal sections stained for Iba1 (green), CD68 (red), and DAPI (blue) from pair-fed (PF), alcohol-fed (EtOH), and CVC-treated alcohol-fed mice, acquired at × 63 magnification. b Quantification of Iba1 and CD68 colocalization, normalized to total Iba1⁺ area per image in the hippocampal CA1, CA3, and DG regions and throughout the HPF. c Flow cytometry data showing the percent of microglia (defined as CD11b⁺ CD45^lo) that are positive for CD68 expression. Data are mean ± SEM, n = 3–4 mice/group with 4–5 images per region per mouse. *p < 0.05; n.s., not significant by one-way ANOVA