Table 1.
Antigen | Color | Clone | Company | Cat. no. | |
CD11b | APC | M1/70 | Biolegend | 101212 | |
CD45 | PE/Cy7 | 30-F11 | eBioscience | 25-0451-82 | |
CD68 | PerCP/Cy5.5 | FA-11 | Biolegend | 137010 | |
CD86 | FITC | ||||
MHC-II | APC/Cy7 | M5/114.15.2 | Biolegend | 107628 | |
CD206 | PE | C068C2 | Biolegend | 141706 | |
CD163 (goat host) | n/a | K-18 | Santa Cruz | sc-18796 | |
Goat IgG | Qdot 525 | n/a | Invitrogen | Q22072 | |
Primary antigen | Primary dilution | Company | Cat. no. | Secondary and fluor | Secondary dilution |
IBA1 | 1:500 | Wako | Anti-Rb-488 | 1:500 | |
CD68 | 1:500 | AbD Serotec | MCA1957 | Anti-Rt-647 | 1:500 |
Secondary antibodies from Life Technologies