Table 2.
Real-time PCR primers
| Forward | Reverse | |
|---|---|---|
| Nanostring hits | ||
| C1qa | CAAGGACTGAAGGGCGTGAA | GGGGCTGGTCCCTGATATTG |
| C1qb | GGTGCCAACAGCATCTTCAC | TTTGACCCCGTGATTACGCA |
| Daxx | CTATAGGCCAGGCGTTGACC | GTTCGATTTTCCCGAAGGCG |
| Gnas | ATGGGTTTAACGGAGAGGGC | ACCATCGCTGTTGCTCCTTG |
| Hmgn1 | CTCCTCGGTGACAGATCCGA | AACCTTCCTCTTGGGCATCG |
| Hspb1 | CTGGCAAGCACGAAGAAAGG | GCACCGAGAGATGTAGCCAT |
| Il-23 | TGGTTGTGACCCACAAGGAC | CAGACCTTGGCGGATCCTTT |
| Map3k9 | TGGGCAGAAAGAGCTCACAT | ACATCATCTGCCTCTTACCCTTC |
| Mapk1 | CAGTTTGTCCCCTTCCATTGAT | ACTCCCACAATGCACACGAC |
| Mef2a | AGCACTTTGAAAGGAAGAGTCCA | AGCTCCCCCACTGCACATTA |
| Myd88 | AGGCATCACCACCCTTGATG | CGAAAAGTTCCGGCGTTTGT |
| Plcb1 | AGATCCTCGATGAGAAGCCC | CTTCCGACAAGACTGAGGAGG |
| Tgfβ1 | GTCACTGGAGTTGTACGGCA | AGCCCTGTATTCCGTCTCCT |
| Itgb2 | TTCCTGGTGCCAGAAGCTGAA | CCCCGTTGGTCGAACTCAG |
| Ccl11 | TGCAGGCAGTTTTCTCTGGA | AGGCTCTCCCGACTAGCTTT |
| Proinflammatory | ||
| Tnfα | GAAGTTCCCAAATGGCCTCC | GTGAGGGTCTGGGCCATAGA |
| Tlr4 | TCAGAACTTCAGTGGCTGGA | AGAGGTGGTGTAAGCCATGC |
| Cox2 | AACCGAGTCGTTCTGCCAAT | CTAGGGAGGGGACTGCTCAT |
| Ym1 | CAGAAGCTCTCCAGAAGCAAT | TGCCAGACCTGTGACAAGAAT |
| Il-1β | TCTTTGAAGTTGACGGACCC | TGAGTGATACTGCCTGCCTG |
| Il-17 | CAGGGAGAGCTTCATCTGTGT | GCTGAGCTTTGAGGGATGAT |
| CCR2/5 network | ||
| Ccl2 | CCACAACCACCTCAAGCACT | AGGCATCACAGTCCGAGTCA |
| Ccl3 | ATATGGAGCTGACACCCCGA | TCAACGATGAATTGGCGTGG |
| Ccr2 | GTGTACATAGCAACAAGCCTCAAAG | CCCCCACATAGGGATCATGA |
| Ccr5 | TGGGGTGGAGGAGCAGGGAG | TAGGCCACAGCATCGGCCCT |
| Housekeeping | ||
| Gapdh | GGCAAATTCAACGGCACAGT | GATGGGCTTCCCGTTGATGA |
| 18S rRNA | GTAACCCGTTGAACCCCATT | CCATCCAATCGGTAGTAGCG |