Table 4.
Gene expression profile of the hippocampus
| Hippocampus | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| PF | EtOH | PF vs EtOH | EtOH + 6wk CVC | EtOH vs 6 weeks | EtOH + 3wk CVC | EtOH vs 3 weeks, p value | |||||
| Mean | Stdev | Mean | Stdev | p value | Mean | Stdev | p value | Mean | Stdev | ||
| Nanostring | |||||||||||
| C1qa | 1.059 | 0.351 | 1.049 | 0.411 | 0.966 | 0.851 | 0.265 | 0.344 | 0.670 | 0.235 | 0.078 |
| C1qb | 1.009 | 0.150 | 1.462 | 0.147 | *< 0.001 | 1.782 | 0.459 | 0.134 | 1.563 | 0.265 | 0.431 |
| Ccl11 | 1.156 | 0.743 | 14.52 | 12.66 | 0.073 | 4.626 | 3.510 | 0.095 | 3.333 | 3.057 | 0.062 |
| Daxx | 1.029 | 0.261 | 0.821 | 0.398 | 0.310 | 0.733 | 0.217 | 0.645 | 0.622 | 0.253 | 0.327 |
| Gnas | 1.017 | 0.208 | 0.585 | 0.306 | *0.017 | 0.613 | 0.100 | 0.834 | 0.492 | 0.226 | 0.563 |
| Hmgn1 | 1.017 | 0.213 | 1.482 | 0.333 | *0.016 | 1.302 | 0.213 | 0.291 | 1.485 | 0.645 | 0.993 |
| Hspb1 | 1.044 | 0.322 | 1.357 | 0.432 | 0.185 | 1.274 | 0.278 | 0.703 | 1.013 | 0.283 | 0.134 |
| Il23 | 1.804 | 1.911 | 10.62 | 5.981 | *< 0.01 | 3.780 | 4.233 | *0.045 | 3.099 | 3.019 | *0.020 |
| Itgb2 | 1.225 | 0.749 | 0.632 | 0.254 | 0.097 | 1.340 | 0.601 | *0.024 | 0.913 | 0.471 | 0.227 |
| Map3k9 | 1.073 | 0.414 | 2.298 | 1.428 | 0.071 | 1.533 | 0.443 | 0.239 | 1.079 | 0.293 | 0.068 |
| Mapk1 | 1.087 | 0.409 | 1.033 | 0.242 | 0.785 | 1.041 | 0.570 | 0.976 | 0.887 | 0.251 | 0.329 |
| Mef2a | 1.022 | 0.233 | 1.636 | 0.702 | 0.070 | 1.577 | 0.183 | 0.846 | 1.784 | 0.349 | 0.653 |
| Myd88 | 1.029 | 0.263 | 1.379 | 0.285 | 0.052 | 1.222 | 0.272 | 0.354 | 1.022 | 0.209 | *0.033 |
| Plcb1 | 1.087 | 0.438 | 1.685 | 0.307 | *0.021 | 1.724 | 0.328 | 0.837 | 1.742 | 0.191 | 0.708 |
| Tgfβ1 | 1.039 | 0.302 | 0.946 | 0.344 | 0.630 | 0.982 | 0.342 | 0.858 | 0.488 | 0.312 | *0.036 |
| Proinflammatory | |||||||||||
| Tnfα | 1.246 | 0.651 | 2.206 | 1.911 | 0.291 | 1.490 | 0.665 | 0.407 | 1.383 | 1.214 | 0.394 |
| Il1β | 2.320 | 0.757 | 2.587 | 1.707 | 0.853 | 1.622 | 0.811 | 0.239 | 1.570 | 0.866 | 0.222 |
| Il17 | 1.069 | 0.696 | 1.017 | 0.534 | 0.859 | 1.652 | 0.745 | 0.121 | 1.213 | 0.769 | 0.620 |
| Tlr4 | 1.047 | 0.236 | 1.772 | 0.856 | 0.083 | 1.773 | 0.891 | 0.999 | 1.496 | 0.728 | 0.562 |
| Cox2 | 1.007 | 0.153 | 1.011 | 0.168 | 0.970 | 1.275 | 0.231 | *0.047 | 1.325 | 0.257 | *0.031 |
| Ym1 | 1.093 | 0.412 | 22.40 | 13.11 | *< 0.01 | 8.849 | 9.192 | 0.084 | 8.183 | 7.048 | *0.041 |
| CCR2/5 network | |||||||||||
| Ccl2 | 1.051 | 0.633 | 3.926 | 2.837 | *0.034 | 3.858 | 2.162 | 0.963 | 1.451 | 1.033 | 0.072 |
| Ccl3 | 1.301 | 0.821 | 6.713 | 3.069 | *< 0.01 | 2.188 | 1.338 | *< 0.01 | 2.737 | 2.238 | *0.035 |
| Ccr2 | 1.024 | 0.402 | 11.67 | 6.247 | *0.010 | 3.958 | 3.253 | *0.035 | 2.955 | 2.310 | *< 0.01 |
| Ccr5 | 1.024 | 0.223 | 3.433 | 1.685 | *< 0.01 | 1.659 | 0.404 | *0.031 | 2.267 | 0.733 | 0.151 |
Gene expression was measured by qPCR. Data are mean or standard deviation (Stdev), n = 5–6 mice/group
*p < 0.05 and significant p values are offset from non-significant values