Figure 8.

Br₂-exposed pregnant mice exhibit progressively increased circulating sFLT-1 (soluble fms-like tyrosine kinase 1) with a concomitant increase of lung wet/dry weight. Non-pregnant and pregnant (E14.5) mice were exposed to air or to Br₂ at 600 ppm for 30 minutes and returned to room air. sFLT-1 levels in the plasma of (A) pregnant Br₂-exposed mice increased 48-hour post-exposure vs air controls and continued to increase until euthanasia at 96 hours. This increase was linear (R²=0.8151, P<0.0001). Similarly exposed (B) non-pregnant mice exhibited no increase at any time point; n=6 to 8; ANOVA. Lung wet:dry weight ratios of (C) pregnant Br₂-exposed mice increased 72-hour post-exposure compared with air controls and continued to increase until euthanasia at 96 hours. Similarly exposed (D) non-pregnant mice exhibited no increase at any time point; n=6 to 8; ANOVA. All data are individual values and means±SEM. sFLT-1 indicates soluble fms-like tyrosine kinase 1.