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. 2020 Oct 7;8:e10059. doi: 10.7717/peerj.10059

Figure 4. Validation of transgenic lines.

Figure 4

(A) PCR amplification of the recombinant gene (M: 2000 DNAMarker, -: negative control, +: bacterial positive control, 1-3: transgenic lines); (B) RT-PCR electropherogram; (M: 2000 DNAMarker, WT: wild type plants, 1-5: transgenic lines). (C) qRT-PCR was used to verify the expression of the SlMYB102 gene, with Actin as a normalization control. The data are means + SD (standard deviation) of three biological replicates analyzed by Student’s t-test; *P < 0.05; and **P < 0.01. Error bars represent standard deviation.