Table 1. Primers and probes that have been recommended by the U.S.CDC to detect SARS-CoV-2 by rRT-PCR.
| Gene target | Description | Oligonucleotide Sequence (5′>3′) | Label |
|---|---|---|---|
| 2019-nCoV_N1 | 2019-nCoV_N1 Forward Primer | 5′-GAC CCC AAA ATC AGC GAA AT-3′ | None |
| 2019-nCoV_N1 Reverse Primer | 5′-TCT GGT TAC TGC CAG TTG AAT CTG-3′ | None | |
| 2019-nCoV_N1 Probe | 5′-FAM-ACC CCG CAT TAC GTT TGG TGG ACC-BHQ1-3′ | FAM, BHQ-1 | |
| 2019-nCoV_N2 | 2019-nCoV_N2 Forward Primer | 5′-TTA CAA ACA TTG GCC GCA AA-3′ | None |
| 2019-nCoV_N2 Reverse Primer | 5′-GCG CGA CAT TCC GAA GAA-3′ | None | |
| 2019-nCoV_N2 Probe | 5′-FAM-ACA ATT TGC CCC CAG CGC TTC AG-BHQ1-3′ | FAM, BHQ-1 | |
| RNAse P | RNAse P Forward Primer | 5′-AGA TTT GGA CCT GCG AGC G-3′ | None |
| RNAse P Reverse Primer | 5′-GAG CGG CTG TCT CCA CAA GT-3′ | None | |
| RNAse P Probe | 5′-FAM—TTC TGA CCT GAA GGC TCT GCG CG—BHQ-1-3′ | FAM, BHQ-1 |