Table 1. Primer sequences for the targeted mouse genes.

	Primer sequences (5′→3′)	Annealing temp (°C)	Reference
b-actin	Forward: gctcttttccagccttcctt Reverse: gatgtcaacgtcacactt	60	Niu et al. (2018)
BDNF	Forward: gcgcccatgaaagaagtaaa Reverse: tcgtcagacctctcgaacct	60	Niu et al. (2018)
NCAM	Forward: gggaactccatcaaggtgaa Reverse: ttgagcatgacgtggacact	60	Niu et al. (2018)
SCF	Forward: ccttatgaagaagacacaaacttgg Reverse: ccatcccggcgacatagttgaggg	60	Niu et al. (2018)
CREB	Forward: ccagttgcaaacatcagtgg Reverse: ttgtgggcatgaagcagtag	60	Niu et al. (2018)
MOG	Forward: aaaacaccctgtggtgaagg Reverse: atcctggttggcagaatcac	60	Niu et al. (2018)
MAG	Forward: gttcctcagctcctcattgc Reverse: ttggggatgtctcctgattc	60	Niu et al. (2018)
MBP	Forward: atccaagtacctggccacag Reverse: cctgtcaccgctaaagaagc	60	Niu et al. (2018)
PLP	Forward: caggctcctgctagaaatgg Reverse: ggtcttcaggagatgcttgc	60	Niu et al. (2018)
c-fosa	Forward: cagagcgggaatggtgaaga Reverse: ctgtctccgcttggagtgta	59.5
TNF-α	Forward: acggcatggatctcaaagac Reverse: agatagcaaatcggctgacg	60	Xin et al. (2014)
IL-1β	Forward: atgaaagacggcaccccac Reverse: gcttgtgctctgcttgtgag	60	Ren et al. (2013)
IL-6	Forward: tgcaagagacttccatccagt Reverse: gtgaagtagggaaggccg	60	Ren et al. (2013)
IL-10	Forward: ggttgccaagccttatcgga Reverse: acctgctccactgccttgct	60	Jaini et al. (2010)
IFN-γ	Forward: tcaagtggcatagatgtggaagaa Reverse:tggctctgcaggattttcatg	60	Jaini et al. (2010)
ZO-1	Forward: gatccctgtaagtcacccaga Reverse: ctccctgcttgcactcctatc	60	Zhong et al. (2010)
Claudin-1	Forward: ggggacaacatcgtgaccg Reverse: aggagtcgaagactttgcact	60	Zhong et al. (2010)
Occludin	Forward: ttgaaagtccacctccttacaga Reverse: ccggataaaaagagtacgctgg	60	Zhong et al. (2010)

Note:

^aThe primer sequences of c-fos is designed by National Center for Biotechnology Information (NCBI) and the referenced gene ID is 14281.