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. 2020 Oct 10;132:110848. doi: 10.1016/j.biopha.2020.110848

Fig. 1.

Fig. 1

HCQ inhibited DC activity stimulated by RA patient serum. (A) The percentage of pDCs and mDCs in peripheralblood of patients with RA (n = 29) was increased when compared to healthy donors (n = 20). *** P < 0.001, **** P < 0.0001: RA patient vs healthy control. (B-D) Purified DCs were obtained from PBMCs of a healthy donor and stimulated by serum of healthy controls (n = 6) and RA patients (DAS28-ESR＞5.1, n = 6), respectively. Our data showed that the ability of migration and maturation of DCs stimulated by RA patient serum was enhanced, manifested as up-regulated expression of CXCR4, CCR7, decreased expression of CCR5 and increased level of CD83, CD86. HCQ prevented the activity of DCs by attenuating the expression of CXCR4, CD86 and IFN-α. #P < 0.05, ##P < 0.01, ###P < 0.001: DCs stimulated by RA patient serum vs DCs stimulated by healthy volunteer serum, * P < 0.05, ** P < 0.01: HCQ + DCs stimulated by RA patient serum vs DCs stimulated by RA patient serum. (HC, healthy control; HCQ, Hydroxychloroquine, MFI: mean fluorescence intensity).