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. 2020 Oct 10;132:110848. doi: 10.1016/j.biopha.2020.110848

Fig. 3.

Fig. 3

HCQ suppressed only CpG ODN 1826 induced BMDC activity but not other TLR stimulators. (A) HCQ attenuated IL-12 secretion only in CpG (the ligand of TLR9) induced BMDCs but not other TLR inducers including Pam3CSK4 (TLR1/2), Poly (I:C) (TLR3), LPS (TLR4), Flagellin (TLR5) and CL097 (TLR7/8). (B, E) HCQ prevented the migration and maturation of CpG activated BMDCs, reflected by decreased expression of CCR7, CXCR4, L-selectin, increased expression of CCR5 and diminished level of CD40, CD80, CD86, MHC II. (C) HCQ inhibited both immature DCs (iDCs) and CpG-stimulated mature DCs (maDCs) transportation/from transporting from the upper chamber to the lower chamber under the chemotactic effect of CCL21. (D) HCQ inhibited the CCL21 production of BMDCs activated by CpG. #P < 0.05, ##P < 0.01, ###P <0.001: CpG-activated BMDCs vs CpG-unactivated BMDCs (Ctrl); * P < 0.05, ** P < 0.01: HCQ + BMDCs stimulated by CpG vs BMDCs stimulated by CpG.