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. 2020 Oct 9;11(10):836. doi: 10.1038/s41419-020-03049-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a METTL3 expression in HGC-27 cells transfected with lentivirus encoding shRNA against METTL3 (shM3) and control (shCTL). b METTL3 levels in MKN-45 and AGS cells transfected with overexpressing plasmid (OE) or empty vector (EV). c, d Percentage of m⁶A content in total RNA in METTL3-reducing HGC-27 and METTL3-overexpressing MKN-45 and AGS cells. e Proliferation curves of METTL3-reducing HGC-27 cells. f Colony-formation assays of METTL3-reducing HGC-27 cells. Representative plates are shown on the left, and quantification on the right. g Proliferation of METTL3-overexpressing MKN-45 and AGS cells. h Colony-formation assays of METTL3-overexpressing MKN-45 and AGS cells. n = 3 for each group in c–h. i METTL3 expression in xenograft tumors derived from stable cell lines transfected with lentivirus encoding METTL3 (lvM3, MKN-45 cells), shM3 (HGC-27 cells), and their corresponding controls (lvCTL and shCTL). j Kaplan–Meier plots of palpable tumor-free survival in METTL3-high (n = 6) and control (n = 6) MKN-45 cell xerographs. k Tumor-harboring mice (top) and xenograft tumors (bottom) 3 weeks post-implantation of METTL3-overexpressing and control MKN-45 cells. l Diameter (left) and weight (right) of METTL3-high and control MKN-45 tumors. Each dot represents one sample. m Ki67 levels of METTL3-high and control MKN-45 tumors. n Kaplan–Meier plots of palpable tumor-free survival in METTL3-low (n = 6) and control (n = 6) HGC-27 xerographs. o Tumor-harboring mice (top) and xenograft tumors (bottom) 35 days post-inoculation of METTL3-reducing and control HGC-27 cells. p Diameter (left) and weight (right) of METTL3-low and control HGC-27 tumors. Each dot represents one sample. q Ki67 levels of METTL3-low and control HGC-27 tumors. Data are presented as mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.