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. 2020 Oct 9;11(10):838. doi: 10.1038/s41419-020-03057-w

Fig. 5. ANKRD2 is a downstream target of miR-205-5p and VENTXP1.

Fig. 5

a KEGG analysis to identify pathway terms enriched in the dysregulated mRNAs in miR-205-5p- knockdown head and neck squamous cell carcinoma (HNSCC) cells. b Relative expression of putative target genes in miR-205-5p-NC-, miR-205-5p mimics-, and miR-205-5p inhibitor-transfected HN4. c, d Expression of ANKRD2 in HN4 and CAL27 cells under different conditions was examined by qRT-PCR (c) and western blotting (d). e The correlation between the VENTXP1 transcript level and the ANKRD2 RNA level was measured in 44 HNSCC tissues. f Relative expression levels of ANKRD2 in xenograft tumors by western blotting. g Luciferase activity in CAL27 cells transfected with luciferase reporters containing ANKRD2-wt or mut. The data are represented as the relative ratios of firefly luciferase activity to Renilla luciferase activity. h QRT-PCR analysis of ANKRD2 levels in HN4 and CAL27 cells and normal oral primary keratinocytes (titled “Normal”). i, j Western blotting and qRT-PCR analyses of ANKRD2 expression in HN4 and CAL27 cells after treatment with ANKRD2 siRNAs or OE vector. k The viability of HN4 cells after transfection with ANKRD2-OE or siRNA was determined using CCK-8 assays. l Colony-formation assays were performed with HN4 and CAL27 cells after transfection with ANKRD2-OE or siRNA. **P < 0.05; n.s. not significant.