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. 2020 Oct 9;11:5091. doi: 10.1038/s41467-020-18907-6

Fig. 4. Siglec ligands on cancer cells and tissues.

Fig. 4

a Heat map of binding of pre-complexed Siglecs to breast cancer cell lines determined by flow cytometry. b Immunofluorescence staining of cancer cell lines BT549, MCF-7, T47D, MDA-MB-468, MDA-MB-231, ZR-75-1, and HS-578T with Siglec-7-Fc WT and R124A. Immunofluorescence results on breast cancer cell lines are representative of three independent replicates. c, d Breast cancer patient tissue cores with a pathology diagnosis of IDC (invasive ductal carcinoma), ILC (invasive lobular carcinoma) and MC (medullary carcinoma) were immunostained with: Siglec-7-Fc WT (c), Siglec-7-Fc R124A (d) and imaged at ×20 magnification. Positive signal is represented by brown coloration from chromogenic staining. Darker staining as represented by increased brown coloration indicates Siglec-7-Fc binding (c) as compared to control (d), while blue staining represents cell nuclei (hemotaxylin stain). IHC results are representative of two independent replicates. Scale bars are indicated as 20 µm with a tissue width of 670 µm.