Figure 4.
Virulence potential of non-hypermucoviscous Klebsiella pneumoniae isolates in a mouse pulmonary infection model. (A) The effect of 1 × 107 colony-forming units (CFU) of AH3176-1, AH1689-2, AH1689-3 and AH1689-3 ampRKL47 complement mutant on survival was assessed. (B) At 24 hours post-infection (hpi), the lungs of infected mice were harvested, and the bacterial burden was determined by CFU enumeration (n = 10; *P < 0.05, **P < 0.01, one-way ANOVA). Data are representative of 3 independent experiments. (C) Uronic acid production of different strains. An unpaired two-sided Student’s t-test was performed. Each data point was repeated three times (n = 3). Data are presented as the mean ± s.e.m. *P < 0.05, ns = no significance. The lungs of mice without infection (D) or infected with ATCC700721 (E) and AH3176-1 (F) were collected and stained with haematoxylin and eosin. All images are at ×40 magnification. Scale bars: 250 μM.
Abbreviation: ara, arabinose.