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. 2020 Oct 6;16:2285–2296. doi: 10.2147/NDT.S265601

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© 2020 Liu and Dong.

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Silencing of NR4A2 increases PC12 cell viability after OGD treatment. (A) NR4A2 in PC12 cells exposed to OGD by RT-qPCR; (B) the protein expression of NR4A2 in PC12 cells exposed to OGD by Western blot. NR4A2 was overexpressed or silenced in PC12 cells; (C) the mRNA expression of NR4A2 in PC12 cells after transfection by RT-qPCR; (D) the protein expression of NR4A2 in PC12 cells after transfection by Western blot; (E) PC12 cell proliferation determined by CCK-8 assays; (F) apoptosis level of PC12 cells by flow cytometry; (G) cell cycle distribution of PC12 cells detected by flow cytometry. All values were expressed as means ± SD from three independent experiments. Unpaired t-test (panel (A and B), one-way (panel (C, D and F)) or two-way ANOVA (panel (E and G) followed by Tukey’s post hoc test was utilized for comparison. *p < 0.05, **p < 0.01 vs PC12 cells transfected with Lv-NC or Scr.