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. 2020 Oct 6;16:2285–2296. doi: 10.2147/NDT.S265601

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© 2020 Liu and Dong.

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miR-652 directly targets Mul1. (A) Targeting mRNAs of miR-652 predicted through bioinformatic websites TargetScan (http://www.targetscan.org/vert_72/), miRDB (http://mirdb.org/index.html) and RNA22 (https://cm.jefferson.edu/rna22/); (B) relative luciferase activity of cells co-transfected with Mul1-MT or Mul1-WT with miR-652 mimic or mimic NC; (C) biotin-labeled miR-652 enriched Mul1 fragment detected by RNA pull-down; (D) Mul1 protein expression in rat brain tissues tested by Western blot; (E) Mul1 mRNA expression in PC12 cells after transfection evaluated by RT-qPCR; (F) Mul1 protein expression in PC12 cells after transfection tested by Western blot. All values were expressed as means ± SD from three independent experiments. One-way (panel (C, D and E)) or two-way ANOVA (panel (B) followed by Tukey’s post hoc test was applied for comparison. *p < 0.05, **p < 0.01 vs cells transfected with mimic NC, Biotin-NC, Lv-NC, NR4A2 + NC, Scr, shNR4A2 + InC or sham-operated rats.