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. 2020 Oct 6;16:2285–2296. doi: 10.2147/NDT.S265601

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© 2020 Liu and Dong.

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Overexpression of Mul1 promotes apoptosis of PC12 cells after OGD treatment. PC12 cells were co-transfected with NR4A2 + shMul1 or shNR4A2 + Lv-Mul1. (A) Mul1 protein expression in PC12 cells after transfection tested by Western blot; (B) PC12 cell proliferation determined by CCK-8 assays; (C) apoptosis level of PC12 cells by flow cytometry; (D) cell cycle distribution of PC12 cells detected by flow cytometry; (E) the protein expression of Cyto C and Bcl-2 in PC12 cells evaluated by Western blot. All values were expressed as means ± SD from three independent experiments. One-way (panel (A and C) or two-way ANOVA (panel (B, D and E)) followed by Tukey’s post hoc test was applied for comparison. *p < 0.05, **p < 0.01 vs PC12 cells transfected with NR4A2 + Scr or shNR4A2 + Lv-NC.