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. 2020 Oct 6;13:9929–9939. doi: 10.2147/OTT.S259336

Figure 4.

Figure 4

NCK1-AS1 deletion inhibited cell biological behavior in GC by sponging miR-137 in vitro. MGC803 and BGC-823 cells were transfected with si-NCK1-AS1+miR-137 inhibitor, si-NCK1-AS1+mi-NC, si-NC+miR-137 inhibitor, or si-NC+mi-NC. (A) Cell viability was measured by CCK-8 assay. (B) Colony numbers were analyzed by colony formation assay. (C) Cell migration was examined using wound-healing analysis. (D) Cell invasion was analyzed with transwell invasion assay. Experiments were repeated in triplicate. *** P < 0.001, ** P < 0.01, * P < 0.05.

Abbreviations: GC, gastric cancer; si-NCK1-AS1, small interfering RNA against NCK1-AS1; si-NC, negative control siRNA; mi-NC, negative control miRNA; CCK-8, cell counting kit-8.