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. 2020 Oct 6;13:9929–9939. doi: 10.2147/OTT.S259336

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© 2020 Li et al.

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Cells stably transfected with sh-NCK1-AS1 were used to establish xenograft models. (A) Tumor volume was calculated every week. (B) After 4 weeks, mice were killed and tumor weight was examined in each group. (C) The expressions of NCK1-AS1, miR-137 and NUP43 were detected in two groups by RT-qPCR. (D) The expression of ki-67 in the in vivo tumors were analyzed via IHC assay. Experiments were repeated in triplicate. *** P < 0.001.

Abbreviations: RT-qPCR, quantitative reverse-transcription PCR; sh-NCK1-AS1, short hairpin RNA against NCK1-AS1; sh-NC, negative control shRNA; wt, wild-type; mt, mutant; miR-137, microRNA-137; NUP43, nucleoporin 43; IHC, immunohistochemistry.