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. 2019 Jun 10;141(26):10481–10488. doi: 10.1021/jacs.9b04858

Figure 3.

Figure 3

Low level of mismatches during copying of RNA templates (A) 3′-GAGAG and (B) 3′-CUCUC into 3′-NP-DNA. (A) The 3′-GAGAG template was copied by primer extension in the presence of 10 mM 3′-NH2-2AIpddC and 3′-NH2-2AIpddT. (B) The 3′-CUCUC template was copied by primer extension in the presence of 10 mM 3′-NH2-2AIpddA and 3′-NH2-2AIpddG. The nucleotide highlighted in red indicates the 3′-NH2-ddC at the end of the primer. Reactions were conducted in 200 mM Na+-HEPES pH 8.0, 25 °C, 50 mM Mg2+. Plots show MS analysis of the full-length extension products (+5) from the 4 h time point. Inset: PAGE analysis of the products of the primer extension reaction. P, primer. See SI Figure S5 for Sanger-type sequencing results.