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Simultaneous chemical copying of a randomized pool of RNA templates into 3′-NP-DNA using 3′-NH2-2AIpddNs in solution, where N = A, G, C, or U. The nucleotide highlighted in red indicates the 3′-NH2-ddC at the end of the primer. Primer extension reactions were carried out using 10 mM activated NP monomers (3′-NH2-2AIpddA, 3′-NH2-2AIpddG, 3′-NH2-2AIpddC, 3′-NH2-2AIpddT), 50 mM MgCl2, 200 mM Na+-HEPES pH 8.0, 25 °C. P, primer.
