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Efficient chemical copying of long mixed-sequence RNA templates into 3′-NP-DNA using 3′-NH2-2AIpddNs, with templates (A) 13 nucleotides, (B) 18 nucleotides, and (C) 26 nucleotides in length. All primer extension reactions were carried out in solution using 10 mM activated NP monomers (3′-NH2-2AIpddA, 3′-NH2-2AIpddG, 3′-NH2-2AIpddC, 3′-NH2-2AIpddT), 50 mM MgCl2, 200 mM Na+-HEPES pH 8.0, 25 °C. P, primer.
