Table 2. Correlation Analysis of Peptide Activity with Relative Differences in CD Spectra at Increasing Temperaturesa.
S. aureus MIC | HDMEC CC50 | hRBC MHC | |
---|---|---|---|
∂ 192 nm | 0.082 | 0.608 | 0.773 |
∂ 208 nm | 0.038 | 0.609 | 0.770 |
∂ 222 nm | 0.096 | 0.537 | 0.739 |
Signal differences (∂) at three characteristic wavelengths comparing spectra recorded at 30–75 °C were correlated with peptide minimal inhibitory concentrations (MICs) on S. aureus SH1000, cytotoxicity on human dermal microvascular epithelial cells (HDMEC CC50), and the minimal hemolytic concentration of human red blood cells (hRBC MHC). Numbers are Pearson product–moment correlation coefficients ranging from 0 (no correlation) to 1 (perfect correlation).