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. 2020 Aug 24;63(11):1559–1569. doi: 10.1097/DCR.0000000000001806

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Copyright © 2020 The Author(s). Published by Wolters Kluwer Health, Inc. on behalf of the American Society of Colon and Rectal Surgeons.

This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal.

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FIGURE 3. — Representative immunohistochemistry and in situ hybridization of normal and colon cancer organoids. A–C, Normal and cancer organoids with immunohistochemistry staining for stem cell markers ALDH1, CD44, and in situ hybrization for Lgr5. ALDH1 is less specific, in this case, whereas CD44 marks a more specific cell population. Lgr5 (RNAScope) marks cells with high specificity at the base of crypts. D–F, Stem cell markers show increased enrichment throughout colon cancer organoids especially for ALDH1 and CD44 (p < 0.005 for ALDH1, p < 0.05 for CD44, p < 0.02 for Lgr5). n ≥ 3 organoids; scale bar = 100 μm.