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. 2020 Aug 24;63(11):1559–1569. doi: 10.1097/DCR.0000000000001806

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Copyright © 2020 The Author(s). Published by Wolters Kluwer Health, Inc. on behalf of the American Society of Colon and Rectal Surgeons.

This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal.

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FIGURE 4. — Representative H&E and immunofluorescence staining of induced pluripotent stem cell-derived colitic organoids. A, H&E of iPSC colitis-derived organoid. Note multilayered nature of epithelia. B, Green fluorescence marks vimentin, a fibroblast marker in the mesenchyme. C, Purple staining for CK19 highlights colonic organoid epithelial cells. D, Both CK19 (purple) and vimentin (green) show the distinct epithelial and mesenchymal populations. n = 3 organoids; scale bar = 25 μm. H&E = hematoxylin and eosin; iPSC, induced pluripotent stem cell.