Fig. 4.

In vitro differentiation of myoblast. To evaluate whether the manufactured myoblast product can differentiate into myotube in vitro, myoblast cells were culture in the MyoCult™ Differentiation Medium according to the protocols provided by StemCell Technologies for 7–10 days. Then the cells were stained with Alex Fluor 488 labeled antibodies (MF20) against the myosin heavy chain to evaluate myotube formation. The cells were mounted with Prolong gold antifade reagent with DAPI and visualized under a fluorescent microscope. Scale bar size 200μm