Table 3.

Construction of the Adipocyte–Macrophage model with IFNγ (AMMI) and vascularized adipose tissue model with IFNγ (VATMI). SFM = serum free medium

Model	Day 0	Days 1–7	Day 7	Days 8–10	Day 11	Day 12	Day 13	Day 21
AMMI	Plating of hASC	ATE medium		SFM	Plating of macrophages, SFM supplemented with ATE	20 ng/ml of IFNγ	Addition of 60 ng/ml IFNγ	Analysis
AMMI without macrophages	Plating of hASC	ATE medium		SFM	SFM with ATE	20 ng/ml of IFNγ	Addition of 60 ng/ml IFNγ
VATMI	Plating of hASC	ATE medium	Plating of HUVEC	SFM	Plating of macrophages, SFM supplemented with ATE	20 ng/ml of IFNγ	Addition of 60 ng/ml IFNγ
VATMI without macrophages	Plating of hASC		Plating of HUVEC	SFM	SFM supplemented with ATE	20 ng/ml of IFNγ	Addition of 60 ng/ml IFNγ