Fig. 3.

Characteristic of terminal differentiated MSNs. a Immunostaining of MSNs derived from 70-day pMSNs (58 days at pMSN stage and 12 days at the terminal stage) the following: OTX2 (red signal), NF200 (green signal), ISL1 (red signal), TUJ1 (green signal), SYN 1 (red signal), MAP2 (green signal), GABA (green signal), SOX1 (red signal), and DARPP32 (green signal). The nuclei were counterstained with DAPI (blue signal). The white arrows indicate SYN 1 point signals. b FACS analyses of ISL1⁺, NF200⁺, GABA⁺ and DARPP32⁺ cells. c Expression of MSN markers SYP, CALB, ARPP21, GAD1, DRD1, DRD2, MAP2, and FOXP2 measured by qRT-PCR (n = 3 per cell type; values for independent biological replicates shown as the mean ± SEM). d Total currents across cell membrane elicited by incrementing command potential protocol (from − 60 mV to + 60 mV in 10 mV increments) next to an I–V diagram with standard deviation plotted. e An example of the functional activity of neurons derived from iPSCs. Spontaneous action potentials recorded by the whole cell patch clamp method. Average rest potential value is − 62.4 mV