Table 1.
Results obtained with different samples and determination of target copies/sample quantified by RT-qPCR
| Sample material | ACFSVd RNA copies/sample (minimum) |
ACFSVd RNA copies/sample (maximum) |
|---|---|---|
| Seeds from symptomatic apple fruit | 6,000 | 220,000 |
| Cotyledon from symptomatic apple fruit | 2,000 | 500,000 |
| Seedling from symptomatic apple fruit | 30 | 400 |
| Seeds (V. album subsp. album) | 50 | 1,900 |
| Plant material (100 mg of V. album subsp. album) | 200 | 1.200 |
| Plant material (100 mg of phloem and leaves) from symptomatic apple trees | 1.5 × 108 | 1.2 × 109 |
| One bud from a symptomatic apple tree | 3.000 | 7.7 × 106 |
| Aphids (M. persicae, D. plantaginea) from a symptomatic apple tree | 100 | 800 |
| Larvae of C. pomonella from symptomatic apple fruit | 148,000 | 154,000 |