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. 2020 Oct 10;11(10):843. doi: 10.1038/s41419-020-03075-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A, B ZNF638 protein level was down-regulated with the treatment of P22077 in SK-Hep1 and Huh-7 cells. C The knockdown efficiency of USP7 by two specific SgRNA (Sg1, Sg2) and their effects on ZNF638 expression were determined by immunoblotting in SK-Hep1 cells; proteasome inhibitor Bortezomib (100 nM 8 h) partially rescued declined expression of ZNF638 in USP7-deficient cells. D P22077 (20 μM 24 h) induced ZNF638 protein inhibition was partially rescued by Bortezomib (100 nM 8 h) in SK-Hep1 and Huh-7 cells. E The half-life of ZNF638 protein in normal and USP7-deficient SK-Hep1 cells was determined by using CHX (100 μg/ml) at indicated time points. F Genetic inhibition of USP7 in SK-Hep1 cells increased ubiquitination level of ZNF638. G The enhanced polyubiquitination of ZNF638 in SK-Hep1 cells according to USP7 knockdown was mainly Lys48 but not Lys 63 linked polyubiquitination. H K48-resistant ubiquitin (Lys48 to Arg48) could reverse ZNF638 expression in USP7-deficient SK-Hep1 cells. I ZNF638 and USP7 were endogenously interacted with each other in SK-Hep1 cells as determined by Co-IP assay. J USP7 and ZNF638 were mainly co-localized in cell nucleus as determined by immunofluorescence in Huh-7 and SK-Hep1 cells. K Schematic illustration of the protein structure of USP7. L Three of flag tagged truncated mutant plasmids (Flag-TRAF, Flag-CD, and Flag-HUBL) and wild-type USP7 (Flag-USP7) were transfected into SK-Hep1 cells, followed by immunoprecipitation using flag antibody and immunoblotting with ZNF638 antibody. Each immunoblotting assay was performed at least three times from independent studies.