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. 2020 Oct 10;11(10):843. doi: 10.1038/s41419-020-03075-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — A The effects of USP7 or ZNF638 knockdown on fructose (20 mM 48 h) induced cleaved-SREBP1C were verified by immunoblotting, using nuclear extracts from SK-Hep1 cells. B The subcellular location changes of SREBP1C upon the fructose induction (20 mM 48 h) with or without USP7, ZNF638 knockdown were explored by immunofluorescence in SK-Hep1 cells. C Genetic ablation of ZNF638 in SK-Hep1 cells reduced phosphorylation levels of mTOR, AKT, and S6K. D Genetic ablation of USP7 in SK-Hep1 cells reduced phosphorylation levels of mTOR, AKT, and S6K. E USP7 but not ZNF638 knockdown could increase the ubiquitination of cleaved-SREBP1C in SK-Hep1 cells. F The decreased cleaved-SREBP1C resulting from USP7 knockdown could be rescued by bortezomib (100 nM 8 h) in SK-Hep1 cells. G The half-life of cleaved-SREBP1C in USP7-deficient SK-Hep1 cells was shortened. H The reciprocal interactions between ZNF638/USP7 and full-length SREBP1C were observed by Co-IP assays in SK-Hep1 cells. I The endogenous interactions between ZNF638/USP7 and cleaved-SREBP1C were determined by Co-IP assays using nuclear lysates of SK-Hep1 cells. J The binding sites of USP7 on SREBP1C were determined by transfecting truncated USP7 mutants (Flag-TRAF, Flag-CD, and Flag-HUBL) into SK-Hep1 cells, following immunoprecipitation with flag antibody and detection of SREBP1C. K The binding of ZNF638 on the promoters of SREBP1C target genes were examined via ChIP assay in SK-Hep1 cells, lanes of IgG and SREBP1C were set as negative and positive control.