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. 2020 Oct 10;11(10):843. doi: 10.1038/s41419-020-03075-8

Fig. 6. USP7 and ZNF638 participate in fructose-induced hepatic steatosis in vivo.

Fig. 6

A, B C57BL/6 mice were fed with standard chow diet or diet added with 30% fructose in drinking water. The fructose group mice were subclassified and disposed to intraperitoneal injection of P22077 (10 mg/kg BW) and GalNAc-ZNF638-SiRNA (2 μg/g BW) every week. The body weight of the mice was weighed every week, and after 4 weeks induction, the liver weight and the TG content of the liver were assessed. C The pathological alterations among different groups were detected by HE staining using paraffin sections; the liver lipids accumulation was determined by oil-red o assay; the expression of USP7, ZNF638, ACACA, FASN, SCD, and SREBP1C among different groups were observed by immunochemistry. D Alterations of USP7, ZNF638, ACACA, FASN, SCD, and cleaved-SREBP1C among different groups were determined by immunoblotting, followed by quantitative analysis. The representative statistical results were performed using one-way ANOVA test and shown as means ± SEM from three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.