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. 2020 Oct 7;12:9725–9734. doi: 10.2147/CMAR.S261828

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© 2020 Wang et al.

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ANLN overexpression promotes doxorubicin resistance in MDA-MB-231 cells. (A) QRT-PCR was performed to estimate the expression of ANLN in MDA-MB-231 and MDA-MB-231/ADM cells. MDA-MB-231 cells were transected with pcDNA3.1-ANLN or pcDNA3.1-NC. (B) QRT-PCR was performed to assess the expression of ANLN in the modified MDA-MB-231 cells. The wild and modified MDA-MB-231 cells were treated with doxorubicin. Normal MDA-MB-231 cells served as control. (C) MTT assay was performed to explore cell viability of the MDA-MB-231 cells. (D) Flow cytometry was performed to estimate apoptosis of the MDA-MB-231 cells. (E) The expression of MDR1 and BCRP in the MDA-MB-231 cells was assessed by qRT-PCR. (F) WB was performed to assess the expression of MDR1 and BCRP in the MDA-MB-231 cells.