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. 2020 Oct 7;12:9741–9752. doi: 10.2147/CMAR.S262215

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© 2020 Wan et al.

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PIN1 is the target of miR-193a-5p in cervical cancer. (A) The expression of indicated genes was detected via RT-qPCR when miR-193a-5p was overexpressed. (B) Interference efficiency of miR-193a-5p was detected by RT-qPCR in C-4-I and C-33A cells. (C-D) The expression of PIN1 was tested via RT-qPCR after miR-193a-5p or FBXL19-AS1 were silenced. (E) The expression of PIN1 in cervical cancer cells was tested through RT-qPCR. (F) RIP experiment was utilized to detect the co-existence of FBXL19-AS1, miR-193a-5p and PIN1 in RISCs. (G) The binding sites between miR-193a-5p and PIN1 predicted by ENCORI. (H-I) Luciferase reporter and RNA pull-down experiments were implemented to prove the interplay between miR-193a-5p and PIN1. (J) The knockdown efficiency of PIN1 was tested through RT-qPCR. (K-L) Colony formation and EdU experiments were carried out to test cell proliferation when PIN1 was inhibited. (M) Transwell experiments were implemented to test cell migration and invasion after silencing PIN1. (N-O) Flow cytometry and TUNEL experiments were utilized to test cell apoptosis when PIN1 was subjected to inhibition. *P<0.05, **P<0.01.