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. 2020 Oct 7;13:9957–9966. doi: 10.2147/OTT.S257951

Figure 2.

Figure 2

Overexpressing miR-525-5p inhibits glioma cell proliferation and induces cell cycle arrest. (A) The mRNA expression level of miR-525-5p in U251 and T98 cells was analyzed by qRT-PCR after transfection. *P < 0.05. (B and C) Cell viability of miR-525-5p was evaluated using the colony formation assay. Data were expressed as the mean ± SEM from three independent experiments. *P < 0.05. (D) Overexpressing miR-525-5p inhibited cell proliferation detected by CCK8 assay. Data were expressed as the mean ± SEM from three independent experiments. *P < 0.05. (E) Overexpressing miR-525-5p on the growth of U251 and T98 cells was examined by EdU assay (original magnification 200 X). Data were expressed as the mean ± SEM from three independent experiments. *P < 0.05. (F and G) Effect of miR-525-5p on cell cycle distribution in U251 and T98 cells. Data were expressed as the mean ± SEM from three independent experiments. *P < 0.05. (H) Western blot analysis of CDK2, CDK4, and cyclin D1 protein levels in U251 and T98 cells after overexpressing miR-525-5p and miR-NC. β-Actin was used as control.