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. 2020 Oct 7;13:9957–9966. doi: 10.2147/OTT.S257951

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© 2020 Xie et al.

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Stat-1 reintroduction reverses the inhibitory effect of miR-525-5p. (A) Western blot analysis of Stat-1, FOXM1, and p-p65 protein levels in overexpressing miR-525-5p or miR-NC U251 and T98 cells. (B and C) Effect of Stat-1 reintroduction on overexpressing miR-525-5p or miR-NC U251 and T98 cells growth rates as measured by CCK-8 assay. Data were expressed as the mean ± SEM from three independent experiments. *, ^#P < 0.05. (D–H) Effect of Stat 1 reintroduction on overexpressing miR-525-5p or miR-NC U251 and T98 cells invasion and migration ability as determined by wound-healing transwell assays. Data were expressed as the mean ± SEM from three independent experiments. *, ^#P < 0.05.