Perturbation in the cytoskeleton and cellular morphology due to AN-treatment. Confocal images of MDA-MB-231 cells stained for (from left to right) nucleus, actin, microtubule. Length of the scale bar is 25 μm. From top to bottom (A) Control, (B) AN-treated cells and (C) PN-treated cells. Quantification of (D)Actin intensity, (E) Microtubule intensity, (F) Cell area and (G) Cell roundness. Statistical analysis was performed using ANOVA with ∗ representing p < 0.05.