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. 2020 Oct 7;40(41):7855–7876. doi: 10.1523/JNEUROSCI.0361-20.2020

Table 5.

Statistical summary for behavioral analyses

Strain, manipulation Transgene Injection site Light delivery Stim paradigm Speed, pre (cm/s)Median ± MAD Speed, light (cm/s)Median ± MAD Fold changeMedian ± MAD Sample size (n, mice) Statistical analysisd
p Valuee (Wilcoxon test) p Valuef (Mann–Whitney U test)
PV-Crea,b ChR2-eYFP GPe GPe Patternedc 2.48 ± 0.64 3.54 ± 0.56 +0.52 ± 0.31 11 0.0049
PV-Crea,b ChR2-eYFP GPe GPe Sustainedc 2.52 ± 0.68 4.47 ± 0.72 +0.79 ± 0.28 12 0.00049
PV-Cre eYFP GPe GPe Patterned 2.88 ± 0.55 3.00 ± 0.86 –0.04 ± 0.25 13 0.84 0.022g
PV-Cre eYFP GPe GPe Sustained 2.82 ± 0.60 2.48 ± 0.50 –0.14 ± 0.20 13 0.17 <0.0001g
PV-Cre sham GPe GPe Patterned 2.06 ± 0.25 2.25 ± 0.38 +0.11 ± 0.20 11 0.28 0.013g
PV-Cre sham GPe GPe Sustained 2.16 ± 0.82 2.25 ± 0.79 –0.16 ± 0.31 9 0.91 0.0075g
PV-Cre, 6-OHDAb ChR2-eYFP GPe GPe Patterned 1.24 ± 0.39 1.84 ± 0.64 +0.48 ± 0.26 14 0.00037 0.89g
PV-Cre, 6-OHDAb ChR2-eYFP GPe GPe Sustained 1.40 ± 0.50 2.57 ± 0.69 +0.72 ± 0.20 14 0.00012 0.82g
Npas1-Cre-tdToma ChR2-eYFP GPe GPe Patternedc 2.98 ± 0.66 1.74 ± 0.56 –0.41 ± 0.10 14 <0.0001
Npas1-Cre-tdToma ChR2-eYFP GPe GPe Sustainedc 3.04 ± 0.56 2.08 ± 0.41 –0.37 ± 0.09 16 0.00024
Npas1-Cre-tdTom eYFP GPe GPe Patterned 2.95 ± 0.41 3.08 ± 0.40 +0.06 ± 0.19 11 0.83 0.00018h
Npas1-Cre-tdTom eYFP GPe GPe Sustained 2.89 ± 0.36 2.74 ± 0.56 –0.13 ± 0.15 10 0.13 0.0089h
Npas1-Cre-tdTom sham GPe GPe Patterned 2.43 ± 0.58 2.52 ± 0.42 +0.02 ± 0.08 6 >0.99 0.0008h
Npas1-Cre-tdTom sham GPe GPe Sustained 3.16 ± 0.23 2.76 ± 0.51 +0.19 ± 0.36 6 >0.99 0.044h
PV-Cre ChR2-eYFP GPe STN Patterned 2.81 ± 0.52 4.34 ± 1.02 +0.41 ± 0.16 10 0.0020
PV-Cre ChR2-eYFP GPe STN Sustained 2.88 ± 0.47 5.26 ± 1.48 +0.84 ± 0.11 10 0.0020
PV-Cre eYFP GPe STN Patterned 3.38 ± 1.09 3.36 ± 1.04 –0.00 ± 0.10 6 >0.99 0.00025i
PV-Cre eYFP GPe STN Sustained 3.77 ± 0.79 3.54 ± 0.53 –0.08 ± 0.03 6 0.31 0.00025i
Npas1-Cre-tdTom ChR2-eYFP GPe dStr Patterned 2.89 ± 0.45 1.85 ± 0.53 –0.29 ± 0.11 12 0.00049
Npas1-Cre-tdTom ChR2-eYFP GPe dStr Sustained 2.66 ± 0.78 2.17 ± 0.62 –0.19 ± 0.14 12 0.034
Npas1-Cre-tdTom eYFP GPe dStr Patterned 2.96 ± 0.85 2.98 ± 1.04 +0.06 ± 0.17 11 0.77 0.0006j
Npas1-Cre-tdTom eYFP GPe dStr Sustained 2.68 ± 0.62 2.86 ± 0.97 +0.07 ± 0.16 11 0.32 0.011j
Npas1-Cre-tdTom GtACR2 GPe GPe Sustained 3.19 ± 0.39 4.37 ± 0.50 +0.34 ± 0.34 9 0.020
C57BL/6J GtACR2 STN STN Sustained 2.67 ± 0.31 4.45 ± 0.60 +0.60 ± 0.16 10 0.0020

aPretrial, basal ambulatory activity: PV-Cre: 2.88 ± 0.61 cm/s, n = 67 mice; Npas1-Cre-tdTom: 2.77 ± 0.54 cm/s, n = 56 mice; p = 0.91 (Mann–Whitney U test). Spontaneous locomotor activity (speed, pre-period): PV-Cre: 2.77 ± 0.61 cm/s, n = 67 mice; Npas1-Cre-tdTom: 2.82 ± 0.44 cm/s, n = 56 mice; p = 0.14 (Mann–Whitney U test). Data from all PV-Cre and Npas1-Cre-tdTom mice used in this study were pooled.

bPretrial, basal ambulatory activity: PV-Cre, naïve: 2.62 ± 0.86 cm/s, n = 12 mice; PV-Cre, 6-OHDA: 1.72 ± 0.38 cm/s, n = 14 mice; p = 0.0037 (Mann–Whitney U test). Spontaneous locomotor activity (speed, pre-period): PV-Cre, naïve: 2.51 ± 0.62 cm/s, n = 12 mice; PV-Cre, 6-OHDA: 1.25 ± 0.35 cm/s, n = 14 mice; p = 0.000056 (Mann–Whitney U test).

cData from patterned and sustained stimulation paradigms were compared for PV-Cre (p = 0.094) and Npas1-Cre-tdTom (p = 0.24; Mann–Whitney U test).

dTwo-tailed p values are listed. The bold p values are significant (i.e., < 0.05). No multiple testing corrections were considered.

eLocomotor speed during pre-period and light-period from the same mice were compared.

fFold changes from different experimental groups were tested. Only data obtained with the same stimulation paradigm were compared.

gData were compared with PV-Cre, ChR2-eYFP, light delivery at the GPe.

hData were compared with Npas1-Cre-tdTom, ChR2-eYFP, light delivery at the GPe.

iData were compared with PV-Cre, ChR2-eYFP, light delivery at the STN.

jData were compared with Npas1-Cre-tdTom, ChR2-eYFP, light delivery at the dStr.