Table 1.
Antisera used for DAB technique and multilabeling immunofluorescence staining
| Group | Primary antisera | Secondary antisera | Tertiary antisera |
|---|---|---|---|
| NeuN/GFAP | Rabbit anti-NeuN (1:200, Cellsignal, catalog #12943S, RRID:AB_2630395) Mouse anti-GFAP (1:200, Millipore, catalog #ab MAB3580, RRID:AB_94936) |
Alexa Fluor 594 donkey anti-rabbit (1:500, Invitrogen, catalog #A21207, RRID:AB_141637) Alexa Fluor 488 donkey anti-mouse (1:500, Invitrogen, catalog #A21202, RRID:AB_141607) |
|
| BDA* | - | - | Avidin-biotinylated peroxidase complex (1:200, Vector, catalog #PK-6101, RRID:AB_2336820) |
| FG/BDA | Rabbit anti-FG (1:500, Millipore, catalog #AB153-I, RRID:AB_2632408) |
Alexa Fluor 488 donkey anti-rabbit (1:500, Invitrogen, catalog #A21206, RRID:AB_2535792) | Alexa Fluor 594 avidin (1:1000, Invitrogen, catalog #A-21135, RRID:AB_2535774) |
| FG/FOS | Rabbit anti-FG (1:500, Millipore, catalog #AB153-I, RRID:AB_2632408) Mouse anti-FOS (1:500, Abcam, catalog #ab208942, RRID:AB_2747772) |
Alexa Fluor 488 donkey anti-mouse (1:500, Invitrogen, catalog #A21202, RRID:AB_141607) Alexa Fluor 594 donkey anti-rabbit (1:500, Invitrogen, catalog #A21207, RRID:AB_141637) |
- |
| FG/ BDA/ FOS |
Rabbit anti-FG (1:500, Millipore, catalog #AB153-I, RRID:AB_2632408) Mouse anti-FOS (1:500, Abcam, catalog #ab208942, RRID:AB_2747772) |
Alexa Fluor 594 donkey anti-mouse (1:500, Invitrogen, catalog #A21203, RRID:AB_2535789) Alexa Fluor 647 donkey anti-rabbit (1:500, Invitrogen, catalog #A31571, catalog #RRID:AB_162542) |
FITC-avidin (1:1000, Vector, catalog #A-2001, RRID:AB_2336455) |
*Nickel-DAB-H2O2 reaction.